Disera-blood

Disera-blood

VACUSERA’s venous blood collection uses evacuated PET tubes and safety needles/holders to standardize draws, reduce needlestick risk, and protect sample integrity. After venipuncture, tubes are filled in the specified order—citrate (incl. CTAD) → serum (CAT ± gel / thrombin+gel) → heparin (± gel) → EDTA (± gel) → glucose → trace element → ACD—then gently inverted (typically 3–10 times, tube-dependent). When required, tubes are centrifuged at the catalogue-specified RCF and times (e.g., serum CAT 1300 g × 10 min; gel/thrombin 2000–3000 g × 10–15 min; citrate 2000–2500 g × 10–15 min). The product line covers routine serum/plasma testing, hematology (K2/K3EDTA), coagulation (3.2/3.8% citrate; CTAD), glucose stabilization (NaF-based), trace elements, crossmatch, and ACD, with options like aprotinin EDTA for ACTH, gel separators, and pre-barcoded labels to streamline ID and minimize preanalytical errors.

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  • System components: PET evacuated tubes + butyl stoppers + PE caps; safety holders; straight needles (18–23G) and butterfly safety sets (21–25G) with optional pre-attached holders; tourniquets; sharps containers.

  • Labeling & ID: Paper, transparent, or pre-barcoded tubes; follow IFU symbols (IVD, EO/irradiation sterilized, latex-free, etc.).

  • General steps (venipuncture):

    • Prepare patient & select site → apply tourniquet.

    • Disinfect, perform venipuncture with holder + needle/set.

    • Order of draw (see below) to avoid additive carryover.

    • Fill each tube to mark (vacuum defines volume), immediately invert per tube requirement.

    • Release tourniquet, withdraw needle, activate safety, dispose in sharps.

    • Label tubes (if not pre-barcoded), transport upright.

  • Order of draw (VACUSERA, venous):

    • Sodium citrate (coagulation/ESR) – 3.2% or 3.8% (incl. CTAD)

    • Serum – clot activator (CAT) ± gel; thrombin + gel (rapid serum)

    • Heparin – lithium or sodium ± gel (plasma)

    • EDTA – K2EDTA/K3EDTA ± gel (hematology / molecular)

    • Glucose – NaF/EDTA or NaF/oxalate

    • Trace element tubes (CAT / K2EDTA / sodium heparin)

    • ACD (blood grouping/crossmatch as specified)

  • Mixing (gentle inversions right after draw):

    • Serum (CAT ± gel / thrombin+gel): 5–6 inversions

    • Citrate (coag/ESR/CTAD): 3–4 inversions

    • Heparin (± gel): 8–10 inversions

    • EDTA (± gel): 8–10 inversions

    • Glucose (NaF + EDTA / oxalate / Na2EDTA): 8–10 inversions

    • Trace element / ACD: follow tube IFU (typically 3–10)

  • Centrifugation (when applicable; RCF × time):

    • Serum CAT: 1300 g × 10 min

    • Serum CAT + gel / Thrombin + gel:2000–3000 g × 10–15 min (or 4000 g × 5 min for thrombin+gel)

    • Citrate (coag):2000–2500 g × 10–15 min

    • Heparin (plasma): 1300–2000 g × 10 min (per tube type)

    • EDTA + gel (molecular):1100–1500 g × 10 min

    • Crossmatch serum (CAT): 1300 g × 10 min

    • Notes: swing-out rotors ~10 min; fixed-angle often ~15 min (per IFU).

  • Tube families (common venous use):

    • Serum: CAT; CAT + gel; Thrombin + gel (fast clot).

    • Coagulation:3.2% / 3.8% sodium citrate, CTAD (citrate + theophylline/adenosine/dipyridamole) to suppress in-vitro platelet activation.

    • Plasma:Lithium heparin or sodium heparin (± gel).

    • EDTA:K2EDTA / K3EDTA (± gel for molecular). K3EDTA + aprotinin for ACTH at room temp.

    • Glucose:NaF + K3EDTA, NaF + K-oxalate, or NaF + Na2EDTA.

    • Specials:Trace element (low-contam), Crossmatch (CAT / EDTA), ACD-A/B (blood grouping).

  • Quality & safety notes:

    • Fill citrate tubes precisely (9:1 blood:additive).

    • Underfilling or poor mixing risks clotting/hemolysis and recollection.

    • Use safety needles/sets; activate safety and dispose immediately.

    • Store/transport per tube shelf life (most 18 months; some 4–12 months) and temperature limits on label.