CFX-96 Touch

CFX-96 Touch

Summary

The CFX96 Dx is an IVD-marked, open real-time PCR platform built on the C1000 Dx thermal cycler with the CFX96 Dx optical module. It offers 5-target multiplexing in a single well with reaction volumes down to 10 µL, factory-calibrated six-channel optics for sensitive, crosstalk-free detection (down to 1 DNA copy), and fast scanning. The system pairs robust thermals (Peltier block, gradient) with CFX Manager Dx software for guided setup, QC, analysis, and reporting—usable as a stand-alone instrument or up to 4 instruments controlled from one PC—suited for clinical assay use or open assay development.

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Main features

  • Capacity & format: 96 wells; 1–50 µL (10–25 µL recommended)

  • Multiplexing: up to 5 targets per well; all-channels and fast-scan acquisition modes

  • Optics: 6 filtered LEDs / 6 filtered photodiodes; 450–730 nm range; reads each well individually; 12 s scan (all channels), 3 s fast single-channel

  • Sensitivity & range: detects 1 copy; 10-log dynamic range

  • Thermals: Peltier; 0–100 °C range; max ramp 5 °C/s, avg 3.3 °C/s; gradient (30–100 °C, programmable span 1–24 °C); accuracy ±0.2 °C @ 90 °C; uniformity ±0.4 °C within 10 s of arrival @ 90 °C; heated lid to 105 °C

  • Software (CFX Manager Dx): startup wizard; standard-curve quantification, melt-curve, allelic discrimination, end-point analysis; ΔCq/ΔΔCq gene expression with multiple reference genes; QC tools; customizable PDF/Excel/Word/PowerPoint exports; email notification with data file

  • Configurations: run as stand-alone or control up to 4 instruments from one computer

  • Connectivity: USB 2.0

  • Footprint & weight: 33 × 46 × 36 cm (W×D×H); 21 kg (47 lb)

  • Regulatory: In-vitro diagnostic (IVD) use; CE-IVD; electrical approvals IEC, CE.