Art04

Art04

Summary
ART04 is a point-of-care, cartridge-based real-time PCR platform that automates magnetic-bead nucleic-acid extraction and multi-channel fluorescence detection inside a “mini-lab” disposable. It supports 200 µL–1 mL input from a wide range of specimens (NP swabs, sputum, BAL, feces, urine, blood, CSF, etc.) and targets bacteria, viruses, mycoplasma, and chlamydia. The instrument offers rapid temperature control (4–105 °C), six dye channels (FAM, HEX, TAMRA, ROX/Texas Red, Cy5, Cy5.5), and multi-color melt-curve analysis. One PC can control up to four instruments, and each instrument can run up to four cartridges in parallel. Current/announced reagent menus cover respiratory panels (FLU/RSV/SARS-CoV-2; Mycoplasma/Chlamydia; MTB/NTM/RIF; MTB/INH/FLQ) with additional kits under development (carbapenem-resistance genes, MRSA/SA, LRTI bacteria, ZIKV/DENV/CHIKV, HPV, and GI pathogen panels).

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 Main features

  • Fully automated magnetic-bead extraction with mini-lab cartridge

  • High-speed multichannel fluorescence acquisition + multi-color melt curves

  • Temperature control: 4–105 °C, accuracy ±0.1 °C; ramp rates up to 2.5 °C/s (heating) and 2.0 °C/s (cooling)

  • Six optical channels: FAM, HEX, TAMRA, ROX/Texas Red, Cy5, Cy5.5

  • Sample volume: 200 µL–1 mL; specimens: NP swab, sputum, BAL, feces, urine, blood, CSF, etc.

  • Throughput/scalability: runs up to 4 cartridges per instrument; cascade up to 4 instruments from one computer

  • Working conditions & I/O: 10–30 °C; network port + USB

  • Footprint/weight: ~350 W × 600 L × 550 H mm, 48 kg

  • Reagent kits: available—FLU/RSV/SARS-CoV-2; Mycoplasma/Chlamydia; MTB/NTM/RIF; MTB/INH/FLQ.
    Under development—Carba-R (KPC/NDM/VIM/IMP/OXA-48), MRSA/SA, LRTI-B panel, ZIKV/DENV/CHIKV, HPV (HR types), GI panels (bacteria/parasites/viruses).

  • Application scenarios: clinical (drug guidance, infections, genetic diseases) and non-clinical (pet viruses, food safety, animal diseases).